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Slices (300-µm thick) were cut on a Leica VT1200S and incubated in cutting solution in a holding chamber at 32°C for approximately 30 min followed by continued incubation at room temperature prior to electrophysiological recording, at which point slices were transferred to a submersion-type recording chamber attached to the microscope stage.
Slices from the dorsal hippocampus were submerged in a holding chamber, and the sucrose cutting solution was gradually shifted to recording solution pre-warmed to 25°C (ACSF in mM: 124 NaCl, 2.5 KCl, 10 glucose, 26 NaHCO3, 1 NaH2.54, 2.5 Cand2, and 1.3 MgSO4, pH 7.4) over 15 20 min in the incubation chamber.
Both cutting solution and ACSF were constantly bubbled with 95%O2/5%% CO2.
The cutting solution contained (in mM): 27 NaHCO3, 1.5 NaH2224, 222 Sucrose, 2.6 KCl, 3 MgSO4, 0.5 CaCl2.
The brains were removed rapidly and placed in the ice-cold and oxygenated NMDG cutting solution described earlier.
After cutting, slices were stored at room temperature for at least 1 h in cutting solution before recording.
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