Sentence examples for cutting restriction from inspiring English sources

According to this method, genomic DNA was digested with two frequently cutting restriction enzymes, which do not cut within telomeric repeats, resolved by pulsed field gel electrophoresis (PFGE), transferred onto a nylon membrane and hybridized with a telomeric probe.

Briefly genomic DNA was extracted from MEFs, digested with the frequently cutting restriction enzymes Hinf1 and Rsa1.

Although this study has relatively few sequence loci available for comparison, our findings suggest that a denser RAD marker scan, using a more frequently cutting restriction enzyme would interrogate more genome sequence and interrogate more sequence for comparative analyses.

We rather expect that this ITS is interspersed by nontelomeric sequences containing sites for frequently cutting restriction enzymes and appear in the gel as fragments below 4 kb like the ITS of birds [ 68].

Based on our positive experience with PstI-based genomic representations [ 25] we initially evaluated several combinations of PstI with different frequently cutting restriction enzymes (RE) as a complexity reduction approach for sorghum.

It is unclear if the BJ1 genome ever forms a circular molecule but if it does then cos sites are unlikely to be involved as digests with three infrequent cutting restriction enzymes (HindIII, EcoRV and EcoRI) followed by melting at 80°C failed to show any change in the number of bands compared to un-melted digests (data not shown).

Before transformation, the plasmid DNA was linearised by digestion with a rare-cutting restriction enzyme, SdaI, that cuts in the minimal vector just outside of the cDNA inserts (Fig. 2).

The DNA to be sequenced is fragmented to 500 bases and four adaptors are inserted into each genomic fragment by repeated cutting with restriction enzymes and intramolecular ligation.

We used previously sequenced individuals [ 24] to identify a rare-cutting restriction enzyme that differed between coastal individuals, which had the cut site, and interior individuals, which did not.

Rare-cutting restriction enzymes are important tools in genome analysis.

For example, to identify the flanking sequences of T-DNA insertions in plants, (Cottage et al. 2001) digested plant genomic DNA with blunt-cutting restriction enzymes and annealed an adapter sequence to the blunt-ended unknown region.