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However, the addition of 500 μM spermine improved the total cutting percentage significantly.
We confirmed that in the absence of Mn2+, essentially no cleavage was seen and that the highest total cutting percentage was observed at 15 mM Mn2+.
The intensity of bands in these reactions was plotted using the 'profile' function of ImageJ [ 46], and the total cutting percentage was quantified as P = 1 - [intact 3× pen aRNA]/[total 3× pen aRNA].
Perhaps not surprisingly, it was also found that longer incubation time greatly improved the total cutting percentage when the same concentration of DNAzymes was added in the reaction mixture (Table 3).
Similar(56)
The company has been cutting the percentage of its American profits paid to the Internal Revenue Service for years, resulting in a far lower rate than at most multinational companies.
According to the Times, "the company has been cutting the percentage of its American profits paid to the Internal Revenue Service for years... based on an aggressive strategy that mixes fierce lobbying for tax breaks and innovative accounting that enables it to concentrate its profits offshore".
Linkage group 4 was also found to harbor a QTL (near marker P_204_ C) responsible for cutting biomass percentage.
On the other hand, a strong correlation between leaf biomass and cutting biomass percentage in year 1 may be due to the linkage of different QTLs located on a similar region of a chromosome (linkage group 4).
a: cutoff point; sensitivity: percentage of malingering and war-related PTSD groups correctly classified at cutting score; specificity: percentage of malingering and war-related PTSD groups correctly classified at cutting score; HR, overall hit rate: percentage of all groups correctly classified.
Similarly, a strong credit shock increases the percentage of firms cutting costs by 16 percentage points relative to those hit only by a fall in demand.
Housing investment plummeted by 16.4% in the year to the first quarter, cutting a full percentage point off GDP growth.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com