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Aligned sequences were clustered into operational taxonomic units (OTUs) defined at 97% similarity cutoff using the average neighbour method.
The second term takes into account the long-range interactions (2 < r < cutoff) using the standard Lennard-Jones potential with a cutoff of 12 Å.
The resultant 49,386 log-transformed probe intensities were filtered by means of a 0.5 variance cutoff using the genefilter method [18] to recover 24,646 expressed probes in at least one sample.
We obtained 2,757,258 reads following adaptor trimming, quality (0.05), and length filtering (50 bp cutoff), using the software package CLC Genomics Workbench 4.5.1.
Additionally, we tested HLTF, HPP1 and NEUROG1 as continuous variables without cutoff using the PMR values and calculated univariate Spearman correlation coefficients.
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This expression was usually of mild to moderate intensity, and when stratified using the cutoff used for the tumors, all were considered negative for SIAH2.
For CA125, receiver-operating characteristic (ROC) curves were calculated, using SPSS SPSS Inc.., 2001), to evaluate the cutoff used and the usefulness of the test.
We used the cutoff used in the previous study; in a sensitivity analysis we tested other cutoffs from day 1 to day 7.
This line indicates the percent recovery at different levels of RMSD diversity when the RMSD cutoff used is the same as the diversity level.
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