Trial Registration: The original trial (van der Heijde et al., Arthritis Rheum 2005) that is the basis for this secondary report had FPI 06-Jan-2000 and LPO 24-Sep-2001, which predate the cutoff requirement for trial registration.
The information about worm paralogs is obtained from the EuGenes database [ 18] and consists of 30036 paralogous pairs involving 10071 worm proteins (blastp with 10-30 cutoff and no requirements on the length of aligned region).
NRS outcome cutoffs were selected based on accepted definitions of "moderate-to-severe" and "mild" postoperative pain [ 8], and the IV morphine requirement cutoff chosen as 1 SD (standard deviation) from mean postoperative morphine requirements in hip arthroscopy as established by Morgenthaler [ 9].
The online 454 Replicate Filter (http://microbiomes.umms.med.umich.edu/replicates/) was used to filter out exact replicate reads (cutoff 0.99, length requirement 1.0 and initial base pair match 3).
We tested, for example, different E-value cutoffs, different length requirements on the match region or when using methods of homology estimation that are completely independent of particular E-value thresholds (Additional file 1).
Parameters were set at 0.95 sequence identity cutoff, 0 length difference requirement, and 3 beginning base pairs to check.
For data sets with <350 Mb of shotgun reads, the online 454 Replicate Filter (http://microbiomes.msu.edu/replicates/ [date last accessed 17 Nov 2011]) was used to filter out exact replicates (cutoff = 1.0, length requirement = 1.00, and initial base pair match = 3).
For the purposes of describing the prevalence of deficiency, we used the computed Estimated Average Requirement (EAR) cutoff for estimated 24-h urinary iodine excretion (160 μg/d, assuming 90% excretion of the EAR of 180 μg/d), as in our previous study in UK pregnant women (8).
The authors performed various sensitivity analyses that involved using a lower threshold (90 th percentile) to define hypertension, use of 30% as cutoff for abnormal load, more stringent requirements for adequacy, and limited non-SLE group to females.
Therefore, this paper presents a verification methodology that reduces a given grid system model to a model to which it is possible to apply a "cutoff" theorem (i.e., a requirement is satisfied by a system with an arbitrary number of jobs if and only if it is satisfied by a system with a finite number of jobs up to a cutoff size).
