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The NICB also advises installing a simple ignition kill switch or fuel cutoff device in a hidden location to make it that much more difficult for a crook to start a car or truck and drive it away.
Each PAT was also fitted with an emergency cutoff device provided by the manufacturer which physically released the tag if it went below 1800 m (which is the maximum nominal safe depth for tag operation).
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Hcy and unreacted Hcy-thiolactone were removed by four cycles of dilution with buffer A containing 2 mM dithiothreitol (DTT) and ultrafiltration using a 10-kDa cutoff Millipore device (10,000x g, 4 °C, 30 min).
Separation of FITC labeled flagellin from unbound FITC was performed by using 30 kDa cutoff ultrafiltration devices (Millipore).
Cell-conditioned media was then collected, filtered and concentrated using either 3 or 30 kDa cutoff bioseparation devices (Millipore).
Using the 10,000, 30,000, and 50,000 kDa cutoff filter devices, we fractionated the whole culture supernatants to <30, 30 to 50, and >50 kDa fractions.
From an analytical standpoint, using double linear halo the sensitivity of channel charge versus the VGS variations (Cg) is decreased, therefore according to (3) this device cutoff frequency is increased.
As required, protein was concentrated to 5 13 mg/mL using an Amicon 30 kDa cutoff centrifugal filter device (Millipore).
The fractions containing MDH were collected and concentrated using a Centriprep molecular weight cutoff (MWCO) 10 device.
Supernatants were pooled across 4 15 donors and concentrated 15-fold using a 3-kDa molecular weight cutoff Amicon ultrafiltration device (Merck Millipore).
The purified enzyme in TE buffer (pH 7.5) was concentrated by using 30-kDa cutoff Centrifugal filter device (Ultracel YM-30, Millipore, Billerica, MA, USA).
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