Suggestions(1)
Exact(2)
This subsequently required exclusion of the plateau cycles from the nonlinear regression, based upon a somewhat esoteric method for selecting a "cutoff cycle" [ 18].
Viral RNA copy numbers were determined from amplification of a standard curve of positive control RNA, and the cutoff cycle threshold (Ct) value for a positive reaction was set at 35 cycles.
Similar(58)
Experimenting with various cutoff cycles has indicated that setting the cutoff one or two cycles above or below this cycle does not significantly affect MAK2 concentration predictions.
After interplate calibration, data were tested for outliers with cutoff SD (cycles) = 0.25 and Grubbs test was performed with confidence level = 0.95.
Using the L90M assay as an example, the resulting distribution of collated ΔCTs from the pre-ARV era wildtype samples supported a ΔCT cutoff of 10.5 cycles for L90M clinical testing (ΔCTs ranged from 12.0 28.0 cycles) (Figure 3).
A negative control (water only) was included for each series of reactions and PCR products were visualised by agarose gel electrophoresis for the conventional PCRs or using a Cq cutoff of 35 cycles for real-time PCR, as described above.
A cutoff of 38 cycles was used to define positive samples.
Moreover, the output Vout is discontinuous since the negative cycle is cutoff.
The cycle threshold cutoff value was 36.
We explored the effect of the parameter value (delta cycle threshold cutoff) in the Xpert system on the determination of rifampicin resistance.
Furthermore, we were able to show that a reduction in BAALC expression levels below the initially defined cutoff after the second cycle of chemotherapy resulted in better EFS.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com