Exact(1)
The obtained UniProt hits were filtered using a cutoff alignment score set at 10% of the hDBC1-hDBC1 alignment score based on trial and error.
Similar(59)
After the initial selection of GSK homologs by annotation mining and BLAST searches, homologs were detected using BLAST (cutoff 30% alignment identity and 80 amino acids alignment length) in selected genomes.
Employing a conservative cutoff for alignment reliability of identical sum-of-pairs score >80% resulted in 56,297 alignments for which we reconstructed maximum-likelihood trees; the remaining 59,453 alignments were excluded because about half (49 ± 16%) of the site patterns (columns) in the alignment were irreproducible in the simplest alignment comparison (N-terminal vs. C-terminal seeding).
Directly related to this is the ratio of the aligned region to the full length of a given contig, plotted against decreasing cutoff value alignment score.
Thus, we compared these lncRNAs with the non-coding portion of our sperm transcriptome dataset by BLAST (E-value = 9e-13, this E-value cutoff allows alignment to have ~60 bp contiguous perfect match).
For sequences with percent identity values close to the 99% cutoff and BLASTn alignment length of <100%, pairwise alignments with the best species-level match were analyzed by using MEGA4.1 (29 ).
We vary the global alignment cutoff distance and show different diversity estimation metrics for cutoffs at 0.03, 0.05 and 0.10.
RepeatMasker was run using an alignment cutoff of 250 (-cutoff 250) and sensitive search (-s).
We used 1e-04 as a cutoff for BLASTN alignment results (either on TREPnr or Repbase).
The fragments with an alignment cutoff value less than 7 were considered as candidate targets.
The E-value cutoff, identity and alignment coverage were set at 10−10, 30% and 40%, respectively.
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