Sentence examples for cut the insert from inspiring English sources

That suggests that a second mutation cut the insert out and restored coloration.

Each plasmid was digested with the appropriate restriction enzyme (Table S3) that cut the insert once, approximately in the middle in order to clone the aacC3-IV gene conferring resistance to gentamycin (GmR) [48].

A total of 10 μg of genomic DNA was digested with EcoRI, which does not cut the inserted intron fragment, and was separated by agarose gel electrophoresis.

C. cellulolyticum genomic DNA was extracted using a Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA): 10 μg of genomic DNA was digested with EcoRI, which does not cut the inserted intron fragment, and was separated by agarose gel electrophoresis.

Just cut the power wire, insert the inline fuse and reattach.

The doctor will cut the skin and inserts the needle to the surface of the kidney.

In particular, restriction enzyme mediated cloning of an insert requires that the cloning enzymes do not cut within the insert.

The recombination step requires digestion of one BAC clone with I- PpoI which should not cut in the insert region.

To resolve a large repeat region, the 2.1 kb insert of a sub-clone containing the entire region with 100 to 150 base pairs (bp) of flanking DNA was excised from pCR4-TOPO with EcoRI (which does not cut in the insert), eluted on an agarose gel and extracted with a MinElute column.

Acc65 I cuts IS4, B3 or UK4 VL sequences at a position in FR2 that is 106 base pairs from the beginning of VL, but does not cut the expression vector outside the insert.

He cut the tangled wires and inserted some wire-and-metal sutures — made from materials on the shuttle following instructions from the ground — to prevent the tear from widening.