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4-µm formalin fixed paraffin embedded sections were cut, placed on SuperFrost/Plus slides (Fisher), and dried overnight at 37°C.
After light microscopy, selected parts were chosen for re-embedding in Epon (see e.g. [28]) and 75 nm sections were cut, placed on grids, and finally counterstained with lead citrate and saturated uranyl acetate.
Ultrathin sections were cut, placed on 300-mesh nickel TEM grids, stained with uranyl acetate and lead citrate, and examined using a JEOL 1010 transmission electron microscope (JEOL Ltd., Peabody, MA) at 80 kV.
Ultrathin sections were cut, placed on nickel grids and incubated in sodium metaperiodate.
Four micron formalin-fixed paraffin-embedded sections were cut, placed on SuperFrost/Plus slides (Thermo Fisher Scientific), and dried overnight.
Tissue sections (10 μm) were cut, placed on glass slides, and stained with hematoxylin and eosin by using standard techniques.
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To record the FE-SEM micrographs, 2 × 2 mm-sized samples of scaffolds were cut and placed on metal stubs using double-adhesive tapes before sputter-coating with gold.
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Ultrathin sections were cut and placed on gold grids.
Tissue sections (5 µm thick) were cut and placed on positively charged slides.
Sections, 3 mm thick, of formalin-fixed, paraffin-embedded tissue were cut and placed on slides coated with 3-aminopropyltriethoxysilone.
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