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The resulted proteins were then compared against the non-redundant database with an e-value threshold cut off set to 0.005 [25].
Negative Predictive Value 52.8% (95% CI,) Cut off set at 0.7.
However, only high correlation coefficients |0.8 < r < 1.0 | with elevated significance (cut off set as 10-4.3 10-4.3considered (see additional file 3).
We have used Cox' proportional hazards model to compare the survival distributions of groups with high and low levels of plasma biomarkers (cut off set to median value), and to obtain estimated hazard rate ratios.
The Infernal options for a default search is given in the CM model files provided by Rfam, and depends on the model with the following parameters as variants: -g (global/local(default) alignment), -T (score cut off, set at the so-called gathering score cutoff for the given model), and –fil-no-hmm (turns of HMM pre-filtering, speeding up the search at the cost of sensitivity).
The signal was recorded at a sampling rate of 100 Hz and anti-aliasing filter was used with a cut off set at 50 Hz.
Similar(49)
Similarity searches were performed on the two databases using BLASTP [ 53] with default settings and a cut-off set to E≤10−30.
Data were high-pass filtered with a frequency cut-off set at 32 s, the duration of the task alternation period, and low-pass filtered using a hemodynamic response function.
Transcriptional responses of the CA1 region with the subsequent Illumina microarray analysis returned a total of 1592 genes that were statistically differentially expressed in KA-treated as compared to the age-matched control animals with the filter cut-off set at the 2-fold difference in the gene expression level with the p-value <0.05.
For example, the upregulated CG34449 gene (Fig. 4A) showed only one statistically significantly upregulated exon at the 3' end, but most of the other probes were also upregulated in 2nd and 3rd instar larvae without reaching the statistical cut-off set in the study.
Anti-PRP antibodies were measured by ELISA with a cut-off set at 0.15 μg/ml.
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