Removing all OTUs with an abundance of less than 100 sequences (an arbitrary cut-off selected after inspection of the data) still yielded a statistically significant difference between seasons for both Bacteria and Archaea (ANOSIM R = 1.0, p<0.01.0,
Potential transcription factor binding sites in the human MER20-derived decidual PRL enhancer (dPRL) were identified using the MATCH program (http://.gene-regulation.com/) utilizing TRANSFAC binding site matrices with a match cut-off selected to minimize the sum of false positive and false negative results.
As all of these programs require a list of differentially expressed genes as input, the analytical results are influenced by the significance cut-off selected by the user.
The cut-off selected to define a positive test for a continuous testing method should be selected to optimise the balance between sensitivity and specificity.
Another cut-off selected to discriminate low or high CD8+ T cell infiltration did not change the absence of correlation between CD8+ T cell infiltration and prognosis (data not shown).
We evaluated the entire data set using different cut-offs for the ADC (data not shown), and the cut-off selected (mean ADC threshold of 1.2 × 10 3 mm2/s) performed best in terms of overall accuracy.
Once the model is chosen, the risk cut-off selected for the screening algorithm will be a trade-off between the sensitivity and specificity, and where this cut-off is set is an economic decision which should ideally be evaluated using decision models.
For example, using a 0.23 ug/ml CAE cut-off (selected to give an age-adjusted positivity rate of approximately 10% in the SDCL control population aged 50 or less) the relationship between the cumulative positivity rate, based on this cut-off, and age for subjects aged <50, 50-59, 60-69 and 70-79 is linear (Y = 0.65X-15.8, R2 = 0.98, Figure 6).
The cut-offs selected were based on previous studies [ 35], done on depressed patients.
Classification of the primary tumors into categories based on protein expression in immunohistochemistry is somewhat subjective, and the optimal cut-offs selected are still being discussed even for proteins such as ER [ 41].
The analysis was performed in two groups as the PIS scoring does not return a continuous variable; the cut-offs selected for each PIS score range resulted in two equal-sized groups (N=20 each).
