Our rigorous classification procedure, employing a set of PFAM domain-specific models and manually curated GA cut offs performs with high specificity and sensitivity, as shown by comparison with data reported for well-annotated Plantae genomes.
Therefore, although the naïve cut-off performed well due to the clearly defined modes and little replication error, the algorithm may perform poorly under different conditions when a range of modes have not been considered [ 30].
High-pass filtering (cut off 128s) was performed to remove low frequency artefacts.
Local TBLASTN with e-value cut off of 1e-6 was performed to search the MEP, carotenoid and bixin pathways genes.
For genes not identified by the above methods, TBLASTN (E value cut off of 0.1) searches were performed using opossum or wallaby sequences obtained from IDMM, against the available Tasmanian devil transcriptomes, and the best hits were used as queries to BLAST the Tasmanian devil genome to determine their genomic location.
While deliberately cut off, they perform acts of generosity — for example, many donate blood frequently.
It was during the EIA when one of the presenters intentionally or not referred to the importance of water while trying to convince the audience of his sincerity: "If we do not perform, cut off our water".
BLASTX (e-value cut off ≤ 10e-10) searchesearcheserformed against Viridiplantae protein database first, then the sequences were no hits were used to performed successive BLagainstainst the NCBI nr protein database in order to make an assessment of the putatiViridiplantae of the sequences.
BLASTX (e-value cut off ≤ 10e-10) searches were performed against a Viridiplantae protein database first.
In order to test the presence of expressed repetitive sequences, BLASTN (e-value cut off ≤ 10e-50) searches were performed against all Viridiplantae Repbase (reference database of eukaryotic repetitive DNA).
Communication with the lander has been cut off for now, as it performs a planned maneuver.
