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BLAT (version 33) was used to align the 'junction exons' from the CGs to the vertebrate genome mRNA and EST sequences using an E-value cut off of less than 10−6.
An E_value cut off of less than 10-4, withethe subject and query databases swapped between runs.
As there is no validation study that provides a cut off score for rating low quality studies, a cut off of less than 5 was used to consider a study as 'low quality' in this review.
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A less rigorous FDR cut off of >0.05 was applied to report SNPs of interest to aid the comparison of results from past and future study populations.
Data was normalized with a significance cut off of p = 0.05 and the false discovery rate of less than 0.05.
In accordance with Wacholder et al [21] a standard FRFP cut-off of less than 0.5 was selected with a cut-off of 0.2 being considered more stringent.
By a less stringent definition, using a reticulocyte cut-off of less then 150,000/uL (∼3% of erythrocytes in a non anaemic child [18]), 86% of all severely anaemic children fell into the RCPF category (table 1).
A false discovery rate cut-off of less than 0.01 was applied for both NTk and NEk statistics for each gene set, within a given genotype comparison (e.g. CbCln6nclf/nclf versus CbCln6+/+ and CbCln3Δex7/8/Δex7/8 versus CbCln3+/+), and the total list of significantly changed gene sets (FDR<.01) are supplied in Tables S1 and S2.
Cognitive impairment will be classified using the MMSE cut-off of less than 24/30.
Using a cut-off of less than 10% risk of 5 year all cause mortality.
Using a cut-off of less than 10% risk of 5 year prostate-cancer specific mortality.
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