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Using this cut off index, we found a better concordance and agreement between the MIF and the SeroCP-IgA tests.
TG-ROC analysis showed that the optimal cut off index for the SeroCP-IgA test, relative to the MIF test, was 1.65.
In comparing the SeroCP-IgA test with the MIF method, we found that the optimal cut off index for the SeroCP-IgA test was 1.65.
The protocol recommends a cut off index value of 1.1 to determine HSV-2 infection.
To normalize the results of different runs, the cut off index for each sample was calculated as sample A450/COV.
Nonetheless, in this study the inter-laboratory variability also occurred in the range around the cut off index value, 1.
Similar(52)
This comparative study highlighted the existence of a grey zone around the cut-off index.
Routine use of the automated chemiluminescent microparticle immunoassay Abbott ARCHITEC T®anti-HBc for diagnosis of hepatitis B is limited in case of borderline reactive sera with low signal close to the cut-off index.
This analysis enabled calculating the approximate project quality index, considering as good-quality projects those that exceeded the 0.50 index (the cut-off index used in the study by Ramos et al. 2010).
In our cohort, the best cut-off index values were 0.4 and 0.8 ((ng/mL)2/1000) to differentiate no AKI risk, high risk and very high risk (Table 4).
Serum antibodies to Herpes Simplex Virus- Type 2 (HSV-2) were detected by ELISA assay (HerpeSelect, Focus Technologies; Cypress, CA), using a cut-off index value of >3.49 to define seropositive specimens.
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