In the case of certain table grape varieties, some clusters are cut off.
Single-linkage clustering was performed with the expectation value cut off of 0.01.
molecular weight cut off.
At the significance cut off, 4 distinct clusters could be recognized [fig 2].
A step-wise increase in the P-value cut off caused the clusters A1, Ch, F1, F2, and M to merge in a supercluster (data not shown), suggesting a close relationship of the enclosed sequences.
For cluster-level correction, the statistical image of uncorrected voxel-wise p values was first cut off by the cluster-forming threshold.
The resulting ALE scores were tested against the earlier calculated 'true' ALE scores and cut off at a cluster-level-corrected threshold of p < 0.05.
A total of 137 operational taxonomic units (OTUs) were detected (using 97% similarity cut off during sequence clustering) and well-known ECM genera such as Cenococcum, Cortinarius, Hebeloma, Inocybe and Tomentella occurred frequently.
The operation unit (OTU) with 97% similarity and clustering cut off the Chimerical sequences was used to identify and exclude with UPARSE (7.1Version http://drive5.com/uparse/).
