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Genes with p-values less than 0.001 and an additional fold change cut off of ±2 were considered statistically significant.
From these normalised entity lists, a significance cut off of p ≤ 0.01 and fold change cut off of ≥ 2 were applied.
Under the assumption that the disease definition changes dependent on the chosen PCR Ct-value cut-off, changes in test estimates for both tests at different PCR Ct-value cut-off for the real-time PCR may be a logical consequence.
Nouvelle cuisine was largely cut off from the changes in the society going on around it.
The optimal cut-off change score was identified as the cut-point with equally balanced sensitivity and specificity [ 89] and this was considered an expression of the MCID.
Thus, the false discovery rate cut off did not change our results.
A gene list was then generated using an FDR (Benjamini Hochberg) [ 18] of 0.05 and a 2 fold cut off for fold change.
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For further analysis, a fold change cut off 1.4 in mRNA expression was applied.
After gene expression data from all groups (hESC, hiPSCs and donor cells) were subjected to the same statistical screening criterion (P-value cut off <0.05 and a fold-change cut off of 2.0), we then calculated the distances among them.
The cut off point for clinically important change in Oswestry Disability index has been proposed as 10 points [ 32].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com