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After perfusion, the brains were removed from the skull, coronarily cut into small blocks, and postfixed in 4% paraformaldehyde overnight.
Sample preparation: Briefly, after dissection, retina were cut into small blocks of about 1mm3, fixed in 2.5% formaldehyde, 0.1% glutaraldehyde in 0.1 M Phosphate Buffer Saline pH 7.4 (PBS) for 1h.
For electron microscopy, 4 brains from 22 24 months old mice were fixed by transcardial perfusion with saline, followed by perfusion and immersion using 2% paraformaldehyde (PFA), cut into small blocks, which were postfixed with 4% PFA and 0.1% glutaraldehyde on ice for 2 h.
The cell pellet was solidified on ice and cut into small blocks.
Jejunum were cut into small blocks and kept for 2 h in this solution for fixation.
After being washed twice with PBS-0.02PBS-0.02%, cells were embedded in 10% gelatin, cooled on ice, and cut into small blocks.
Similar(52)
After perfusion, the brains were removed from the skull, cut coronally into small blocks and postfixed in 4% paraformaldehyde for overnight.
The internal lamellar matrix of cuttlefish bone was cut into small uniform blocks and kept submerged into simulated body fluid (SBF) to form a hydroxyapatite layer on the surface (CB-HA scaffold).
Separate the tofu into smaller blocks.
Grab a block of your favorite cheese and cut into small pieces, then place them in the bowl.
Cut into small dice.
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