Sentence examples for cut downstream from inspiring English sources

Outside of the retail sector, Chevron announced on Jan . 19that it would cut downstream jobs as margins in the market sector were becoming prohibitively thin.

First, mtDNA was digested using an enzyme with a recognition site upstream from OH (Fig.  3B, AflII) in combination with a series of enzymes that cut downstream of OH in the mtDNA major arc (Fig.  3B, XhoI, BamHI, HindIII, PmeI, PstI, BglII).

Step 5: The product was digested with BtgZI, which cuts downstream of its recognition site.

As a Type IIS restriction enzyme, BsmBI cuts downstream of its recognition site, allowing scarless cloning of the gRNA sequence.

(C ) The homology-directed repair (HDR) donor DNA is a 183 nt ssODNA that is complementary to the target sequence (−strand) and contains a 9 nt insertion (HindIII and SphI restriction sequences) at the cut site and a 9 nt deletion downstream of the cut site; these modifications are flanked by 85 nt and 55 nt asymmetrical homology arms at 5′ and 3′ ends, respectively.

An adaptor with an MmeI recognition site was ligated to the 50-end, followed by MmeI digestion that cut 21-22 bases downstream.

The junction of Illumina adapter 1 and CATG site contained the recognition site of MmeI, which cut 17-nt downstream of the recognition site (CATG) to produce tags.

The restriction enzyme, MmeI, was used to cut 21 bp downstream from the recognition site, thus creating a 21 bp unique tag.

Use of Cut, a direct downstream target of Notch signals, as a readout of Notch activation revealed that its activation by Deltex can be suppressed by coexpression of Shrub.

Amplicon sizes were determined by taking the chromosomal location of each SNP on the microarray chip and finding the nearest upstream and downstream cut sites for the Xba nuclease.

After precipitation of the 3' cDNA fragment, Illumina adaptor 1 was added to the 5' end; this adaptor contains a recognition site for the endonuclease MmeI to cut 17 bp downstream of the recognition site (CTAG) and produce 17 bp tags with adaptor 1. Illumina adapter 2 was introduced at the site of MmeI cleavage after removing the 3' fragment via magnetic bead precipitation.