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In addition, FeCl3 was incorporated only in the well cut diffusion agar plate of E. amylovora strain EGY1 DSM 101800 for qualitative detection purposes of the produced siderophore.
Whilst, the last test encompassed the incorporation of FeCl3 (at a final concentration of 100 µM) in the agar plate of E. amylovora strain EGY1 DSM 101800 prior employing this plate in the well cut diffusion test (mentioned below).
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The oxide formation, including some "mushroom-shaped oxidation", is predicted via a combination of thermodynamics and kinetics influenced by the preferential diffusion of specific species using short-cut diffusion paths.
Estimating the siderophore activity of the bacterial isolate S33 was carried out by well-cut diffusion test.
The well-cut diffusion technique was used to test the ability of the crude extracts of sponges to inhibit the growth of indicator bacteria and Candida.
The present simple qualitative assay using FeCl3 in well-cut diffusion assay is a cheap efficient alternate to the currently universal applied assay; Chromazurol-Shuttle assay.
Cell free supernatants were collected separately and were stored at 4 °C till being tested for siderophore activity by well-cut diffusion assay as mentioned above using E. amylovora strain EGY1 DSM 101800 as an indicator strain.
Further, absence of zone of inhibition in E. amylovora strain EGYI DSM 101800 well-cut diffusion assay in presence of 100 µM FeCl3 indirectly emphasized that the antimicrobial agent of quest is a siderophore in nature.
The cell free supernatant was kept at −20 °C till being used in assessment of siderophore activity in further experiments through well-cut diffusion test as mentioned above using E. amylovora strain EGY1 DSM 101800 as an indicator strain.
To verify that the gene encoding siderophore trait is harbored on pSID/EGYII or not, these transformants were tested against E. amylovora strain EGY1 DSM 101800 using well-cut diffusion test.
Previously, this antimicrobial agent was proved to antagonize the growth of E. amylovora strain EGY1 DSM 101800, a fire blight plant pathogen, through the well-cut diffusion assay (Embaby et al. 2014a).
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