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Proteins found in one cut band are grouped, and all are ordered by their respective scores.
Our concern in this experiment was the presence or absence of the small doubly cut band.
The library was then size-selected on a 6% PAGE gel with the cut band corresponding to ∼90 120 bp.
After electrophoresis, the allele band series expected for Ddel polymorphism: for T allele: 261 bp cut band, for C allele: 228 bp and 33 bp two separate bands.
To each cut band was added 400 μl 1× STOP buffer, 1 μl glycogen and incubated in a 68°C water bath until the agarose was completely melted.
The result of COBRA using the collected cells showed the only faint cut band which appears when the AciI recognition site is methylated while the control somatic tissue (kidney) showed similar intensities of the cut and uncut bands as expected, suggesting that the somatic cell contamination in the collected cells was limited even if all the cut band was derived from somatic cells.
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Citigroup, he said, is "trying to fix a problem cut by cut, Band-Aid by Band-Aid".
The PCR band is 972 bp and the theoretical cut bands are 286 bp and 686 bp.
Fig. 3 a Phylogenetic tree with cut bands: Band 1, Band 2, Band 7, Band 10; isolated strains: VF01, VF02, VF03, VF04; and their closest relatives derived from GenBank data based on 16S rRNA gene analysis.
Uncut bands correspond to unmethylated DNA while cut bands correspond to methylated DNA.
A ratio was calculated with the added intensities of the cut bands (methylated) over the intensity of the primary band (unmethylated).
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