Sentence examples for customized vector from inspiring English sources

Both the derived (CMM) and the ancestral (RVT) forms were ligated into a customized vector (pcDNA3-CFP) that includes the cyan fluorescent protein (CFP) coding sequence 3' to the multiple cloning sites, to obtain C-terminally CFP-tagged proteins.

The genes were then inserted into a customized vector (originated from pEYFP from Clontech, Takara, Mountain View, CA) with a CMV promoter and mCherry tag.

For cloning of luxR gene sequences harboring diverse synthetic RDS sequences under the control of the lac promoter, we constructed a customized vector containing the RSF replication origin and lacI q gene.

The disadvantage of the Creator Splice system is that it requires an additional donor vector and customized acceptor vectors.

Therefore, we developed a custom vector arrow plotter from scratch to allow us the flexibility of customizing the look and behavior of the plotted arrows.

This trial established that rationally designed AAV2.5 vector was safe and well tolerated, lays the foundation of customizing AAV vectors that best suit the clinical objective (e.g., limb infusion gene delivery) and should usher in the next generation of viral delivery systems for human gene transfer.

HEK293T cells were plated in 24-well plates at 8×104 cells/well and cotransfected using Lipofectamine 2000 (Invitrogen), with 500 ng pMir target vector customized for ENTPD5 3′UTR (OriGene Technologies, Rockville, MD, USA) and 250 ng pRL-TK (Promega, Milan, Italy) following the manufacturers' instructions.

The researcher only needs to customize the exchange vector and introduce this in the ESCs (Fig. 2C and 3).The modified ESCs are subsequently used for the production of chimeric mice by injection into pre-implantation embryos.

The integrated learners are customized versions of the RT, Support Vector Machine (SVM), CvBoost and Artificial Neural Networks (ANN) implementations in OpenCV and the PLS algorithm in PLearn.

Crossmatch (version 0.990329) and customized Perl scripts were used to trim vectors, adaptors, polyA-ends and low quality nucleotides.

Sequence-based statistics were derived through a customized feature extraction program and fed as a vector for each polymorphism to the J48 classification tree available in the WEKA classifier package.