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Expression of NFAT mRNA was determined using a customised gene expression card (Applied Biosystems) and real-time qPCR was carried out using the ABI Prism 7900HT sequence detection system.
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Taqman arrays customised for potential PCa progression markers were used to study gene expression in a pilot series of 29 patients.
Gene expression was normalized against expression of the GhEF1α gene.
Comparison with published adult mouse (>6 weeks old) expression data was performed using the Bgee gene expression database [ Bastian et al., 2008; release version 12; http://bgee.unil.ch/bgee/bgee; stage MmusDO 0000040; customised, mouse-specific data release (personal communication Frédéric Bastian, University of Lausanne, Switzerland)].
Considering gene expression consistency (the number of single cells in which a gene was expressed), gbM genes had a significantly higher gene expression consistency than unmethylated genes.
Gene expression.
This suppresses gene expression.
dependent gene expression.
J.L. performed gene expression analysis.
Analysis of differential gene expression.
Single cell gene expression datasets.
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