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To our opinion this is due to the goal of the custom target developers design their target to be better optimized for their specific conditions of usage and to maximize the yields.
A custom target consisting of a CrAl half and a MoS2 half in form of triangles was used, which leads to a variation of the chemical composition of the deposited coatings dependent on the substrate position within the coating chamber.
Targeted regions were captured using the Agilent SureSelect custom target enrichment kit (Agilent Technologies, Santa Clara, CA) following Agilent protocols.
We have validated this method on > 150 libraries using custom target designs ranging from 200 Kb to 1.1 Mb and the whole exome (50 Mb).
A total of 3,968,794 sequences from the P. densata mRNA transcriptome database and 38 mature miRNAs were used as a custom target database and a custom miRNA database respectively.
A total of 57,151 unigenes from S. europaea mRNA transcriptome database were used as a custom target database, whereas 210 conserved and 31 novel mature miRNAs were used as a custom miRNA database.
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In addition to the custom targeted enrichments, we also examined depth of coverage for the targeted regions obtained through exome sequencing.
Custom targets have the potential to reach higher yields in comparison to the standard off the shelf compact target (systems).
Every time a user visits one of these sites from the same computer she uses to access Mixi, software from Revenue Science, of Bellevue, Washington, recognizes this and issues a kind of ID tag that allows for custom targeting ("behavior match") of ads on Mixi.
Furthermore, as is the case with most custom targeted resequencing projects, the restricted amount of genome space being covered results in the replication of numerous start and stop positions by chance.
A custom, targeted gene capture was designed using the Agilent SureSelect Target Enrichment kit (Table 3).
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