It charges $5 per templated basic slide or $20 per custom slide, and businesses can pay for subscriptions ranging from $100/month to $1,400/month.
The DNA was enriched for the selected exons by hybridization with the custom slide and sequenced using the SOLiD version 5500 system (Life Technologies).
The leaves were mounted between a custom slide and coverslip, submerged in 400 μL of distilled water and then sealed with petroleum jelly to prevent evaporation.
NP film samples were inserted into standard size (1 cm path length) disposable plastic cuvettes containing custom slide holders that positioned the NP film samples at various angles relative to the beam path of the instrument.
Using the same NP film sample with a 1-PE spacer layer, we collected NP film extinction spectra in T-LSPR mode using a spectrophotometer with the samples inserted into the custom slide holders contained inside of water-filled cuvettes, which positioned the sample at various illumination angles.
Reflectivity was measured using a variable-angle spectroscopic ellipsometer (VASE), and transmission measurements were made using a UV vis spectrophotometer and custom slide holders to support NP film samples inside standard, 1 cm spectrophotometer cuvettes such that the angular orientation of the sample could be controlled relative to the incident light.
All treatments presented here were also tested on whole leaves using the custom slide method of Wertman et al. [ 22] to ensure that the same cell death morphologies occurred in a whole organ and so comparisons could be drawn to the previously described lace plant developmental PCD pathway.
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Custom cDNA slides were spotted with Oligator "MEEBO" mouse genome set with 38,467 cDNA probes (Illumina, Inc., San Diego, CA), which allows interrogation of 25,000 genes.
The custom cDNA slides contained ~10,000 genes (Additional file 2).
PDB oversaw the development and printing of custom microarray slides and helped with the microarray analysis.
