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A custom made script was written to calculate the grid box of the ligand PDB files by taking the maximum and minimum values of the x, y, and z coordinates and adding a total of 15 Å per each axis.
The filtered mate-paired reads were paired again using a custom made script.
The analysis was performed with R (R Development Core Team 2013) using a custom made script.
The coverage of the alignment was estimated and the over-low/high covered sites were discarded by a custom made script to avoid incorrect mapping introduced by random factors or piling up of reads from duplicated genomic regions.
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Custom made scripts were used throughout in order to parse and analyse the data and derive results.
The statistics of the identified SNPs was calculated and evaluated using custom made scripts.
De novo functional annotation of the European eel transcriptome was obtained by similarity using BLAST, Blast2GO and custom made scripts.
The automation was set up on a Magnatrix TM 1200 Biomagnetic Workstation (NorDiag ASA, Oslo, Norway) which is equipped with a 12 tip head and is capable of running custom made scripts.
The annotated genes were classified based on gene-ontology, domain specificity, protein family and the pathways they featured in from UniProt Knowledge Base (UniProtKB) (26) using a custom made perl script (Uniprot_to_functional_annotation.pl; https://github.com/CEG-ICRISAT/cicarvardb-scripts).
Insert size was estimated based on a preliminary assembly by a custom made perl script.
Bacteria were identified by forward and side scatter and data was analyzed in a custom made MatLab script.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com