Using a custom made program written in Matlab (v 6.5, The MathWorks), including the threshold ODBA values described above, the behavioural time budgets of the toads while in the field were calculated.
The custom made program employed to measure size and distance of retinal hemorrhages was developed in MATLAB (R2007a, The MathWorks Inc., Natick MA; U.S.A ., and accounted for different magnification between the cameras and image distortion due to different angles while taking images of the fundus periphery.
Currents were low-pass filtered at 5 kHz, collected using PClamp 9 (Molecular Devices, Sunnyvale, CA, USA) at a frequency 10 kHz and analyzed off line using Clampfit (Molecular Devices, Sunnyvale, CA, USA), ORIGIN (7.5, Origin Lab corporation, MA, USA), and custom made program in MATLAB.
Transformation and analysis were implement by C# language (Visual Studio.NET, Microsoft) using a custom made program.
Accelerometer data were processed by the custom made program Propero 1.0.17 (University of Southern Denmark).
Exploratory bouts were scored using a personal computer running a custom made program written in Visual Basic 6.0 (Microsoft).
A custom made program was developed in C# language (Visual Studio.NET, Microsoft) to implement the method of digital motion analysis developed by [ 20].
Online data was collected with a custom made program in the LabView environment through a digital acquisition board (DAQ, National Instruments, Austin, TX, USA).
Peaks (amax in Fig. 7C) and troughs (amin in Fig. 7C) of the waveform were identified automatically in the custom made program.
