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In order to improve the fluorescence background-to-image ratios, one-photon confocal fluorescence images of the fixed cells were obtained using a custom made filter cube (Ex: 377/50; DM: 409; Em:525/40) for the dye-doped SiNPs.
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Microplate readers equipped with custom made filters may lead to higher fluorescence readings, but to exclude the contribution of excitation wavelengths in the emission wavelength range of EGFP excitation filters with a maximum at 460 470 nm instead of 480 490 nm should be used for red-shifted EGFP variants.
Additional custom made filters were used to remove false positive variant calls, and filters were calibrated by the concordance of sequence and Bovine High Density Chip genotypes and the rate of opposing homozygotes for sire-son paired genotypes.
fEPSPs were 10 × preamplified (custom made preamplifier) filtered at 1 kHz and sampled at 10 kHz using a HEKA double patch-clamp EPC 10 USB amplifier (HEKA Elektronik Dr. Schulze GmbH).
To map polyA-sites to WS190 worm annotations, raw sequence reads were filtered using custom made Perl scripts.
The suspension was extruded, first through a polycarbonate filter with 400 nm pores, followed by passages trough 100 nm pores filters in a custom made extruder.
The filtered mate-paired reads were paired again using a custom made script.
A custom made forward detection mount with an attached photomultiplier tube (PMT) (Hamamatsu, H9305-04), and a band pass filter (transmittance = 475 - 485 nm) was employed to detect the SHG signal.
Before, they were custom made.
"Everything has to be custom made".
Moreover custom made implants are being used.
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