Sentence examples for custom made assay from inspiring English sources
Three tSNPs were selected through HapMap for the IL6 gene −573 G/C (rs1800795) and −174G/C (rs1800796) in the promoter were genotyped as previously described [7], and 3437C/G (rs2069840) in the 3rd intron (Figure 1B, top panel) was genotyped by Taqman with a custom made assay purchased from Applied Biosystems ABII).
A custom made Infinium HD Ultra Assay protocol [ 30] was used for hybridization onto a BeadChip.
The status of the new pagA 981 site was also analyzed in a panel of isolates containing 89 diverse multiple-locus, variable-number, tandem-repeat analysis genotypes and an additional 154 A.Br.008/009 subgroup (Eurasian) isolates by using a custom made real-time PCR assay: TaqMan Minor Groove Binding probes and primers for SNPs (Applied Biosystems) (14 ).
To prove that MDM2 is a direct target of mir-660, we performed a luciferase reporter assay using commercial custom made 3′ UTR MDM2 and observed a strong downmodulation (87% reduction) of the luciferase activity when HEK-293 cells were cotransfected with mir-660.
For a multi-parametric test of probe choice strategies for genotyping with ASH-based assays, we combined custom made (Agilent) high-density microarrays with an in-house multi-stringency array washer which has been described in [13].
Levels of mRNA were investigated using custom made TaqMan Array Cards (Life Technologies) containing 47 gene expression assays including assays for the reference genes, or by using single assays and a previously documented procedure [ 15] (Additional file 1).
However, the requirement of non-commercial custom made BAC probes for FISH testing renders this assay virtually inaccessible to nearly every pathology laboratory around the globe.
Electrophoretic mobility shift assay: Single stranded oligonucleotides were ordered custom made (Sigma) and annealed in annealing buffer over night.
ChIP-reChIP assays were performed using anti-Myc antibody first (Cell Signaling Technology) and then using anti-GFP antibody (custom made).
Before, they were custom made.
T.T.: Do you usually have your suits custom made?
