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The components of the custom informatics system are presented in Additional file 1: Table S1.
A custom informatics system for study fieldwork called Dashboard was developed to improve the speed and completeness of data and sample collection.
The resulting requirement to invest significant resources into custom informatics support for genome sequencing projects remains a major impediment to the accessibility of high-throughput sequence data.
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For the mutation analysis, the Illumina 1.5+ format (fastq) reads were filtered using a custom informatic pipeline (http://tinyurl.com/barcode-tool) that divided them based on barcode.
Proteomes were downloaded from the National Center for Biotechnology Information "nr" database (http://www.ncbi.nlm.nih.gov, last accessed March 28 , 2014 using custom, in-house informatics tools.
At the same time, new graphical user interface-based workflow tools offer facile alternatives to custom scripting for informatics and data analysis.
Using custom developed bio-informatics tools the sequence reads are clustered, aligned, and mined for mutations or single nucleotide polymorphisms (SNPs).
Resulting sequences are clustered and aligned, and the alignments are mined for SNPs using custom-developed bio-informatics tools.
Probesets from this dataset were linked to GPCR annotations and phenotypes from UniProt and the Mouse Genome Informatics databases, respectively, using a custom Python script.
WAD wrote custom python codes for data analyses, performed the bio-informatic analyses of the data and wrote the manuscript.
We, the flow informatics community, basically had to reverse engineer commercial software file formats and write custom open-source software that can read these [ 37].
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