Sentence examples for custom assay from inspiring English sources

Primers and probes for TaqMan assays come from the Applied Biosystems bank (Assays ID: rpl13: Dr03119261_m1; klf4: Dr03080183_m1; Dio3: custom assay AJ20SMU).

TaqMan primer-probe reagents were obtained through the Applied Biosystems Assays-by-Design Assays-by-Design Assays-by-Design, custom

These control samples were run alongside PCR-amplified samples using the original Taqman custom assay (rs227091).

A custom assay was designed for HOXA11 (Forward: biotin-5'-AGAGGTAGGTAGGGAAGATG-3' , Reverse: 5'-CCCCTCCCATAAACTTACTCTAAA-3' , Sequencing: 5'-ACACTCTCTCATTCATAATC-3').

Theoretically, 99% coverage should be obtained by using a higher multiplex custom assay of 1,536 SNP.

The custom assay was optimized against bisulfite sequencing as described [ 56], resulting in the conservative technical threshold of Dm ≥ 0.15 for methylation detection.

Then, a subset of the individuals in several of the crosses were genotyped using custom assays derived from RAD-seq loci that flank either side of the supergene.

Individual offspring from crosses 1, 2, 8, 9, 10, 11, 12, and 13 were genotyped using custom assays derived from RAD-seq loci that flank either side of the supergene, using RAD06/RAD11 on one side and RAD09 on the other side (Table 3; Supplementary Table 2).

These systems combine the ability to sequence gene panels and small genomes, as well as exomes, transcriptomes, and even custom assays on a single platform.

Assays were performed in quadruplicate in 96-well plates with 5 ng of DNA per reaction (for the custom assays,10 ng were used).

Two custom assays were specially developed, one for the Tag SNP and another for rs11713643 (Applied Biosystems).