Exact(2)
The mixture was laid on top of a sucrose cushion (1 ml 20 mM Tris/HCl, pH 7,4/200 mM sucrose, 100μl 20 mM Tris/HCl, pH 7,4/1 M sucrose), centrifuged for 45 min at 50000 × g at 4°C and liposomes were collected from the bottom.
Supernatants were supplemented with Triton X-100 (final 1%) and 40 U/ml RNaseOUT (Invitrogen) and equal volumes (0.5 ml) loaded onto a sucrose cushion (1 M) and polysomes isolated by centrifugation at 100 000 × g for 2 h at 4 °C.
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1 | FOX CUSHION £72.50, Donna Wilson.
Nigella Cyclamen velvet bolster cushion, £40; Nigella Cranberry velvet bolster cushion, £40.
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A floral floor cushion (21 inches square) is $40 at www.anthropologie.com or (800 309-2500.
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The snow on the ground can swell from nothing to a cushion 12ft deep.
Whoopee Cushion, £3, John Lewis 14. 'Private Eye: The First 50 Years', £25, Waterstones 15.
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