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Plates were read on Victor X instrument and the values were analyzed by a four-parameter logistic equation over a 12 point dose response curve (Prism, GraphPad Software, version 5.03, La Jolla, CA).
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Mantel-Cox log rank tests were used to analyze survival curves (Prism, Graphpad).
Concentration-response curves (CRC) were fitted to a non-linear sigmoid regression curve (Graphpad Prism 5.0).
The Kd and Bmax values were calculated from nonlinear curve fits (Prism 4, GraphPad Software, San Diego, CA).
Each melatonin profile was smoothed with a locally weighted least squares curve (GraphPad Prism, San Diego, CA).
Data were fit to a hyperbolic response curve using Prism Graphpad software.
The specific binding data fit best to one site binding (hyperbola) curve (Graph Prism).
The half-life of the displacement curves was determined by fitting the curve with Prism software (Graphpad).
The half maximal activation concentration (AC50) was determined from the sigmoidal dose response curve using Prism 4 (Graphpad, San Diego, CA, USA).
As shown in Additional File 7, the updated model has a higher area under the curve than PRISM.
Data were analyzed using nonlinear regression to a sigmoid curve with Prism 4 (GraphPad Software, Inc., LaJolla, CA).
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