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The amount of product was determined relative to a standard curve of input chromatin.
The relative value of DNA fragments was calculated by extrapolation from a standard curve of input DNA dilutions.
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In contrast, the Logan plot does not require that the shape of the peak be perfectly estimated because it relies on the total area under the curve of the input function.
Perfusion data were processed using a single value deconvolution algorithm with delay and dispersion correction with cerebral blood flow and cerebral blood volume being determined by the peak height and area under the curve of the input residue function, respectively, with mean transit time calculated as the ratio of cerebral blood volume to cerebral blood flow (Bivard and Parsons, 2012).
Figure 6(a) presents signal intensity time curves of arterial input functions (AIF) of all three measurements in the aorta using the BPCM. Figure 6(b) shows the corresponding maps of the time-dependent relaxation rate changes.
Using standard curves, 1% of input was compared to the IP sample and the values from the IgG negative control were subtracted as background.
An example of calibration curve of temperature versus input electric power are presented in Fig. 3.
Y denotes the y values of input curve, and Y' is the normalized curve.
Relative enrichment was determined from a standard curve of serial dilutions of input samples.
Product was measured by SYBR Green fluorescence in 20-μL reactions, and the amount of product was determined relative to a standard curve generated from titration of input chromatin.
To derive functional maps of the perfusion parameters, the arterial input curve of the CM concentration is required, and we obtained this arterial input curve from a region of interest (ROI) in the aorta.
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