Correct molar masses were obtained when the PMMA calibration curve was corrected with data from polyamide blends using a simplex algorithm.
All reported resolutions are based on the gold-standard FSC = 0.143 criteria (Scheres and Chen, 2012), and the final FSC curve were corrected for the effect of a soft mask using high-resolution noise substitution (Chen et al., 2013).
The image-derived blood curve was corrected for partial volume and spillover to produce the actual arterial whole-blood activity A, which was calculated with the following formula: A = left({A}_0-{a}_1* Tright)/{a}_2.
Blood samples (0.5 mL each) were drawn through a port connected to the femoral artery at 15-s intervals until 2 min, followed by 1-mL samples at 3, 5, 10, 30, 60, 90, and 120 min. The plasma time-activity curve was corrected for the fraction of unchanged radioligand following the procedure detailed above.
Because the samples were previously diluted, the concentrations read from the standard curve were corrected by the dilution factor.
The fluorescence change at 338 nm was measured for an average of 60 s at each titration step, and the subsequent titration curve was corrected for ligand without CR.
As explained in the "Methods" section, in the calculation of the scoring function, the impact of any given sub-segment of the AD curves is corrected for the amount of data it is associated with.
Reported resolutions are based on the gold-standard FSC = 0.143 criterion (Scheres and Chen, 2012) and Fourier shell correction curves were corrected for the effects of soft masking by high-resolution noise substitution (Chen et al., 2013).
The curves were corrected to 31° and 27° with the correction rate of 15%and22%2%, respectively after application of the brace.
