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Decline curve analysis.
Finally, a melting curve analysis was performed.
Amplified products were visualized by fluorescence melting curve analysis.
Decline curve analysis is one of the reserve estimation methods.
Melting curve analysis was performed to evaluate primer set specificity.
Simonsohn calls the method "p curve analysis".
A melting curve analysis followed.
Specificity was verified by melting curve analysis.
Melting curve analysis confirmed primers specificities.
Additionally, ROC curve analysis [20] was performed.
Melt curve analysis was performed after each run.
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