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Currently, major challenges facing human organoid technology include (i) improving the range of cellular heterogeneity for a particular organoid system, (ii) mimicking the native micro- and matrix-environment encountered by cells within organoids, and (iii) developing robust protocols for the in vitro maturation of organoids that remain mostly fetal-like in cultures.
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Health care systems in many developing countries, such as Brazil, currently face major challenges: how to meet the demand for 21st century standards of health care and technology with funds that remain lower than what developed nations invested 30 years ago.
However, real-time DNA sequencing is currently a major challenge because longitudinal current detection cannot distinguish individual nucleotides due to the thickness of membrane (>10 bases) and the fast translocation of a single base [45].
Currently, the major challenge in IC treatment is the development of effective therapies.
Vascularization in bone tissue engineering constructs is currently a major challenge.
Thus, the understanding of MM biology is currently a major challenge to eventually cure the disease.
Automatically managing, updating and upgrading the software on these devices, particularly the network stacks, with new, improved functionality is currently a major challenge.
Currently, the major challenge for LICs has been to improve the energy density without sacrificing the high rate of power output performance.
Efficient prediction of the substrate noise generated by large digital sections is currently a major challenge in System-on-a-Chip design.
Currently a major challenge facing efficient plant genetic transformation is in delivering biomolecules through the rigid, multi-layered cell wall of plants.
It also follows that in international terms Chinese people are on average wealthier than we previously thought, though of course this tells us nothing about the level of inequality in China, which is currently a major challenge for its authorities.
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