However, there is currently no highly sensitive system available for the detection of keratinases.
Grade B. Patients with an unstimulated Tg level between 0.1 and 1 μg/l measured using a currently available highly sensitive Tg assay will require at least one TSH-stimulated Tg measurement to assess their disease status.
The large discrepancies seen are most likely due to differences in technique, sample collection, different cohorts and, in particular, the use of currently available highly sensitive PCR and line blot assays with amplification steps.
Molecular genetic testing of mutations in the BRCA1 and BRCA2 genes is currently performed using highly sensitive but labour-intensive direct Sanger sequencing of individual exons.
Among the "novel" biomarkers, which are not currently widely measured, the highly sensitive C reactive protein has the largest evidence base (77 prognostic studies).
This could sometimes be attributed to the sensitivity of the currently used assays; using a highly sensitive IAsys biosensor assay, Salama et al. [ 23] confirmed the presence of anti-α3 chain of type IV collagen antibodies in both patients with crescentic GN and intense linear IgG staining along the GBM who had undetectable antibodies by both ELISA and western blot methods (100%).
SLN mapping using QDs overcomes the limitations of currently available methods and provides highly sensitive, real-time image-guided dissection, which may permit potential mapping of SLNs and lymphatic flow in patients.
These antibodies are currently being used in a highly sensitive competitive ELISA to quantitate levels of BP-DNA adducts in various animal and human tissue samples.
However, no technique is currently able to provide the highly sensitive and highly repeatable measurements needed to establish a diagnostic definition of vascular reactivity during light stimulation of the diseased eye.
Similar to the results from previous studies [ 52, 81], our results show that evolutionary timescales calibrated under currently available molecular clocks are highly sensitive to the choice of calibrating nodes.
