Results from current-control experiments show that changes in rotation rates have no effect on the observed behavior regardless of anion composition or whether the limiting state is stationary or oscillating.
In current-control experiments, the presence of fluoroborate ions causes a substantial increase in the value of the applied current density for which low valued stationary potentials remain stable.
Plasma current control was experimented in the RFX machine in early nineties with unsatisfactory results due to the frequent onset of unstable regimes leading to increasing voltage demand and saturation of the flat-top power supply.
Average amiloride-sensitive current density in control experiments (without treatment with CytD) was 342±57 pA/pF (n = 14).
Quantification of current inactivation from control experiments (described above; Fig 6D, left side) shows the working range of inactivation in this assay: from a median of 0.03 (n = 13) in cells transfected with EGFP-KV3.4 alone (no inactivation/TEVp activity) to a median of 0.65 (n = 14) in cells transfected with EGFP-KV3.4 and RFP-TEVp (maximum inactivation/TEVp activity).
To further substantiate this, we show in Additional file 1: Figures S2 and S3 ion current traces recorded in control experiments (i.e., the absence of bacterial cells), demonstrating the absence and, respectively, presence of CMA3-α-HL reversible interactions, as seen at negative and positive transmembrane potentials held across the nanopore, similar in absolute values to those used herein.
Our current study provides additional control experiments; indeed we determined (1) the effects of the F508del-CFTR mutation relative to the FVB/129 wild-type mouse; (2) a possible influence of sex; and (3) the effect of the DMSO vehicle, all being crucial to validate this technique as a preclinical assay to study CFTR function in this CF mouse model.
To ensure that an adequate ejecting current was used, we performed control experiments with 4-barrel glass pipettes in which we first applied GABA iontophoretically with a current which was sufficient to inhibit a unit's response to its BF, and then ejected BIC with a current which antagonized the GABA-induced inhibition.
Two control experiments without current were also carried out.
As a result, the reliability of the results that derived from the current tightly controlled experiment is considerably high.
(PNG 29 KB) 11671_2013_1547_MOESM3_ESM.pdf Additional file 3: Figure S3: Control experiments on DWNT membrane to rule out redox current.
