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Plasmid curing also occurred in vivo in infected zebrafish with curing efficiency of 17% for nanoparticle + meropenem treatment.
Mortar containing NWPA expand less than that with expanded clay under sealed condition, which suggests the lower internal curing efficiency of NWPA.
The results (Fig. 1D) revealed that plasmid curing due to PtNP treatment occurs from 3 h with a curing efficiency of 17.19% (Table 3).
The curing efficiency of meropenem + PtNP in vivo ranged around 17% (Table 4).
Pore structure and volume water absorption influence the internal curing efficiency of aggregates.
To evaluate the internal curing efficiency of CBBA, the autogenous deformation and internal relative humidity of mortar with pre-wetted CBBA were observed at an early age.
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A total of 18 and 33 thiamphenicol-sensitive colonies, corresponding to curing efficiencies of 50% and 92%, were identified in the three- and seven-transfer approaches, respectively.
To determine the molecular basis of these differences in curing efficiency, we first monitored the levels of Sup35, Hsp104, Ssa1/2 (Hsp70), and Sis1 (Hsproteinsteins, which have all been implicated in Sup35 amyloid fragmentation (Cox, 1965; Chernoff et al., 1995; Song et al., 2005; Higurashi et al., 2008; Tipton et al., 2008; Derdowski et al., 2010).
Thus, curing efficiency correlates directly with the asymmetric retention of Hsp104 in cells at elevated temperature.
Thus, the accumulation of protein aggregates correlates directly with curing efficiency at the various temperatures.
Importantly, treatment with Triton-X-100 over the time course did not result in plasmid curing and frequency of appearance of plasmid lost, small colony variant in Triton-X-100 treatment was comparable to that arising spontaneously in untreated control with curing efficiency ranging at ~1% by 24 h (Table 3).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com