Of these, 57 were manually curated (Additional file 4 and Additional file 5) as the remainder were found to be either allelic variants of the same P450 gene or contained too many sequencing errors.
Of these, 27 were manually curated (Additional file 4 and Additional file 6) as some of the original sequences were found to be either allelic variants of the same CCE gene or contained too many sequencing errors, and 14 were found to be full length.
All analyses were performed using custom Biopython scripts [ 32] and the resulting dataset was manually curated (Additional File 2).
While peptiDB is a set of 103 curated PDB files for non-redundant protein peptide complexes, PepX contains 1431 non-redundant X-ray structures clustered based on their binding interfaces and backbone variations.
Of these food-relevant chemicals, 4719 had curated structure definition files amenable to defining chemical fingerprints, which were used to cluster chemicals using a selforganizing map approach.
All entries in the web-database were exported to a file and curated manually.
(PDF 3507 kb) Additional file 3: Curated ordered gene lists from microarray experiments performed with skin-associated cell types.
The files were curated to remove interaction detection methods that were neither Y2H nor PCA, such as TAP-Tag, mass spectrometry and genetic interactions.
Of note, allele-supertype peptides (i.e., peptides predicted to strongly bind more than one allele with an IC50 below 500 nM) were curated in the output files but were not visualized on the heat maps in this study.
A shift in thinking is needed so that useful data are not simply buried away in supplemental information or in additional files but curated in a suitable repository such as TreeBASE (http://treebase.org/) or, where an appropriate data-specific repository is not available, a generic repository such as Dryad (http://datadryad.org/).org/
