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Unlike ERMA method [ 12], RMEAM doesn't require radioactive labeling of DNA samples and subsequent cumbersome purification steps of the radiolabeled products in tubes.
Even though ERMA can determine overall methylation level within a region containing a number of CpG sites, but it requires radioactive labeling of DNA samples and subsequent cumbersome purification steps of the radiolabeled products.
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Other purification steps are added, including solvents that destroy the lipid shells of viruses like H.I.V. or West Nile.
(A) Purification steps followed by SDS-PAGE.
HPLC chromatograms at different purification steps.
To minimize sample loss during purification, we optimized the experimental procedure and avoided unnecessary purification steps.
Figure 1 shows results of the CBM-SUMO purification steps.
JB proposed the purification steps which SiS and SGK prepared.
The purification steps were repeated for five times.
All purification steps are summarized in Table 2.
All purification steps were designed to be easy.
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