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In this review, three main stress response mechanisms in O. oeni during culturing process were addressed.
After the analysis of several differently mechanical environments comparing with that in vivo, the directly dynamical compression environment, instead of hydrostatic pressure or microgravity or direct perfusion, can recreate the in vivo mechanisms of mechanosensation, mechanotransduction and mass-transport during engineered bone-like tissue culturing process in vitro.
In the enrichment culturing process, we transferred about 10 mL (20%) of the inoculum into a 120 mL autoclaved serum bottle containing 40 mL of sterilized basal medium, while the bottle was flushed with pure N2 gas after passing through copper filings to remove traces of oxygen.
Second, the possible use of low amounts of DNA from any kind of (uncultured) tissue diminishes the technical failures due to the in-vitro cell culturing process [29].
Numerical methods are employed to solve the equations involved, with a focus on investigating the effect of various factors such as culturing time, porosity, and flow rate, which are controllable in the scaffold fabrication and culturing process, on cell cultures.
Fetal bovine serum, or FBS, is an essential ingredient in the culturing process.
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Would the whole loading and column culturing processes affect the fitness and lifespan of cells?
In the co-culture process, DFB and PFB are mixed and cultured in one system.
The cell culture process was the same as described above.
A robust 15-day co-culture process was developed and used in two independent laboratories.
It sums up nicely the symbiotic relationship between culture, process and architectural design.
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