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Chlamydia culturing, preparation of EB stock, and estimation of inclusion forming units (IFU /ml were conducted as previously described [47].
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Mice were killed after 5 days for cell culture preparation and retinal cells were cultured for 24 h.
We thank Mr. Paul Kulvi, Ms. Nancy Copper, Ms. Barbara Chickering, and SPC Edmond Flores in the Department of Pathology for initial specimen culturing and preparation.
The microscopic examination of Gram's stained culture preparation of DAB-1Y as rods in chains and DAB-1W as small cocci bacteria (data not shown).
He committed much of his spare time to BCG culture preparation and developed the first batch of BCG vaccine in China.
The culture preparation was done using such variables as different microbial methods and incubation periods to obtain maximum cells of P. aeruginosa for myrcene biotransformation.
After seed culture preparation, a 2% cell suspension of 0.7 optical density (OD) at 600 nm, which corresponded to approximately 107 colony forming units (CFU) mL−1, was inoculated into 500 mL baffled flasks containing 100 mL MSM.
For the culture preparation, Ethanol Red 11 strain was refreshed by streaking onto YPD agar plate (1% Yeast extract, 2% Peptone and 2% Glucose, 2% agar), incubated for 2 days at 35 °C.
More detailed information about organotypic culture preparation can be found supplementary information (Text S1).
We thank Esther Yu and Rujun Kang for their assistance in neuronal cell culture preparation.
Most surprisingly, the phase of PER2 oscillations in Vipr2−/− SCN is reset by culture preparation.
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