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No toxicity reactions were observed during the culturing period.
Our results indicate that cells stay viable in all the glass extracts for the whole culturing period, 14 days.
Diffuse near-infrared fiber-optic Raman spectra were measured from live cell cartilaginous TE constructs over a 56-day culturing period.
Even after a long-term culturing period of 19 days, we found that the cells on bioactive membranes formed tight monolayers, while cells on non-active membranes lost their monolayer integrity.
Cell proliferation, gel contraction and elastic modulus of the constructs were measured on the same gels at multiple time points during the 22 day culturing period using multiple non-destructive techniques.
Figure 1 Dynamic changes of O3 concentrations in the CF and NF growth chambers during the culturing period.
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Culture medium was changed once during the culture period.
The total culture period was 24 h.
The cells proliferated during the culture period.
The longitudinal modulus is 21 36 GPa which depends on the culture period.
Conversely, D1 cell viability obviously decreased for a further prolonged culture period exceeding 14 days.
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