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These results indicated that fibroblasts could grow into the scaffold bulk using the present seeding and culturing methodologies.
These results indicated that fibroblasts could survive and grow well on the scaffold using the present culturing methodologies.
This contrast in results may have been attributed to the number of patient rooms and surfaces investigated in addition to different sampling and culturing methodologies used among the studies.
Patients with stable vitiligo can be helped surgically using transplantation of autologous cultured melanocytes, but there is a need for a culture methodology that is free from xenobiotic agents and for a simple way of delivering cultured melanocytes to the patient to achieve pigmentation with good wound healing.
There are inherent challenges to optimize an appropriate tissue culture methodology that can aid in enrichment of chondrocytes.
If standard culture methodology is used for future human tissue-engineering, unknown risks of infection from bovine disease or immune reaction to foreign proteins theoretically might occur.
This study provides a basis for the development of a new HF perfusion culture methodology to overcome the limitations of nutrient diffusion in the culture of large 3D tissue constructs.
While certain viruses have been known to have the ability to kill cancer cells, it is only from 1990 to 2010 that technological advances in viral culture methodology and molecular engineering have made clinical translation possible.
In an attempt to create a novel, entirely autologous tissue-engineered adipose substitute, we extracted human stromal cells from either lipoaspirated or resected fat, and assessed their capacity to produce a three-dimensional adipose tissue using an adapted "self-assembly" culture methodology.
In this regard, it is critical to not abandon culture in favor of non-culture methodology, but to apply both modalities simultaneously to all stool specimens.
The latter studies have been hampered greatly by the absence of facile cell culture methodology for this clinically important virus [17], [47].
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