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To induce differentiation, the mouse ES cells were cultured in ES cell culturing media for six days without LIF (Leukemia inhibitory factor).
The earthworm species were then added with partially degraded cow dung as culturing media (Singh and Kalamdhad 2013).
Scientists at the University of California, Davis in the early 1980s tried to mass-produce truffle mycelium, growing them in culturing media without trees.
Another typical role for the SME partner would be to head the refinement of enabling assets such as culturing media, up-scaling technologies for undifferentiated and differentiated cells, as well as derivation of new hPSC lines of GMP grade.
Before the addition of the culturing media and the earthworms, 10 cm-thick bedding for the earthworms was prepared from partially degraded chopped hay (about 50 mm), cattle manure, banana pulp (chopped about 50 mm) and tree leaves.
Isolated cells were plated and maintained in culturing media containing 2 µg/ml of dsRNA or 20 nM of siRNA for 12 to 24 hours prior to recording.
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