Sentence examples for culturing bone from inspiring English sources

As a result, it appears necessary to develop methods to increase the number of MSCs by culturing bone marrow (BM) cells in vitro, mainly to achieve consistent control of cell adhesion and proliferation processes at the biomaterial interface.

Meanwhile, the good cytocompatibility of these composite scaffolds was evaluated by culturing bone marrow derived mesenchymal stem cells (BMSCs) on them.

Culturing bone marrow cells over a period of 4 days resulted in a mixed cell population, which probably also contains few HSCs and MSCs.

Partially differentiated myeloid cells were expanded by culturing bone marrow cells in GMCSF and IL-4 as described for BMDCs for 48 hours.

Macrophages were prepared by culturing bone marrow cells in DMEM medium (Gibco) supplemented with 10% L929 conditioned medium, 10% FBS (BioWhittaker), 100 units/ml of penicillin, 100 mg/ml of streptomycin, and 2 mM glutamine (complete DMEM) for 7 days.

In principle, culturing bone cells is not that easy.

Bone marrow-derived dendritic cells (BM-DCs) were generated by culturing bone-marrow cells from the femur and tibia of 7 10 week old mice in cIMDM media with 10 ng/ml GM-CSF and 20 ng/ml IL-4 in 5 ml of complete medium, with half of the medium replaced by new medium containing GM-CSF and IL-4 on day 2 and 5. On day 6, cultures were added OVA at 1 mg/ml and/or α-GalCer at 200 ng/ml.

When 5 ng/mLlbFGF was added to culturing bone-marrow-derived MSCs, the percentage of mineralized area was maximized at 89% [ 34], and 5 ng/mL FGF increased proliferation [ 38].

We procured a >97% pure population of CD34−CD11c+ DCs by the classical method of culturing bone-marrow cells in the presence of granulocyte macrophage colony-stimulating factor (GM-CSF).

An additional antibiotic, gentamycin (50 μg/ml), was added to the media when culturing bones or co-culturing bones and tumor cell lines.

Their biocompatibilities were also evaluated by culturing human bone marrow stromal cells on the scaffolds.