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Outside another patient's room, he had a group of interns and residents palpating their own thighs as he showed them a technique for finding the right place to stick the needle when culturing an abscess.
The surviving cells were counted by culturing an appropriate dilution of the bacterial suspension in triplicate on agar medium (TSA) for 24 48 h at the appropriate temperature.
Briefly, biogenic Mn oxides were produced by culturing an isolated Mn-oxidizing bacterium using Leptothrix discophora medium with initial Mn II) concentration at 1.0 mM.
Culturing an aliquot of the clinical archival sample number 2 under optimum culture conditions for C. jejuni (in light of the 16S rRNA data) resulted in florid bacterial growth with morphological characteristics typical of C.jejuni.
Alternatively, P. aeruginosa may not be as abundant in the CF airway as indicated by culturing, an idea supported by 16S rDNA and Terminal Restriction Fragment Polymorphism (T-RFLP) analysis of bacteria in CF sputum and bronchoalveolar lavage fluid [33] [35].
Interestingly, culturing an ALL cell line in a low glucose medium or with addition of 2-DG slowed down or inhibited growth but did not induce cell death per se as compared with Dex treatment.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com