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Semi-continuous cultures, which could be periodically harvested at a reasonably high growth rate, were successfully created.
Both compounds showed rapid uptake by A. fumigatus in iron-deficient cultures, which could be blocked with excess of ferri-siderophore and/or sodium azide and was significantly lower (P < 0.05) in iron-sufficient media.
On the contrary, an extensive hemicellulose loss was observed in these cultures, which could be due to the unspecific degradation of this polymer through Mn2+-mediated oxidative reactions [ 32].
Finally, although high PCT levels may detect a sub-group of patients with positive blood cultures [ 7], the clinical relevance of this finding is uncertain and would not eliminate the need for drawing blood for cultures, which could be the only way to identify the microorganism.
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Furthermore, their ships didn't even land at Ellis Island; the organizers didn't want to tempt the immigrants with the taste of anything resembling their own culture, which could be found in oversaturated enclaves like the Lower East Side.
We also need data around retention and culture, which could be included in a diversity report, so that we can continue to hold individual companies accountable for what goes on inside their offices.
We show a method that can allow one to more closely recapitulate this microenvironment in 2D cell culture, which could be applied to any type of cell culture assay.
In contrast, detection of luminescence within the middle ear cavity appeared to be more sensitive than culture, which could be related to the presence of a subpopulation of adherent cells that would be recovered by lavage less efficiently.
Another explanation was the so called "heteroresistance" that means the presence of mixture of susceptible and resistant subpopulation in culture which could be an obstacle against the sensitivity of molecular drug resistance testing and the successive therapy[ 21].
These data demonstrate that CD4+ T cells were capable of cytokine production but do not exclude the possibility that CD4- antigen-presenting cells such as monocytes, B cells and dendritic cells could also produce these cytokines during the culture, which could be secreted into the supernatant.
EBV-infected nasopharyngeal epithelial cells are rapidly lost in culture, which could be attributed to both the rapid loss of EBV episomes in infected nasopharyngeal epithelial cells and growth inhibition after EBV infection [31], which may explain the infrequent rate of EBV infection detected in normal nasopharyngeal epithelial cells.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com